Establishment and Optimization of Micropropagation System for Southern Highbush Blueberry

The optimization of micropropagation for blueberries is crucial due to the growing blueberry industry and demand plantlets. This study focused on four stages: explant sterilization, in vitro establishment, proliferation, ex rooting, aiming establish an efficient propagation system southern highbush cultivar ‘ZY09’. most effective sterilization method was a 60 s treatment with 75% ethanol 5 min 4% NaClO. During establishment culture, modified woody plant medium found be suitable. replacement NH4NO3 (NH4)2SO4 facilitated proliferation microshoots. optimal combination growth regulators microshoots indole-3-butyric acid (0.1 mg·L−1), thidiazuron (0.0005 zeatin (1 mg·L−1). Perlite suitable substrate rooting. best rooting performance observed without immersion regulators. Indole-3-butyric enhances root formation suppresses elongation blueberries. findings this can applied large-scale provide reference genetic transformation